Identification of aspartic acid 52 as the point of attachment of an affinity label in hen egg white lysozyme.
نویسندگان
چکیده
Hen egg white lysozyme, fully inactivated by the affinitylabeling reagent, the 2’,3’-epoxypropyl fl-glycoside of di-(NaCetyl-D-glUCOSamine), contained 1 mole of the label per mole of protein. Total enzymatic digestion of the reduced and carboxymethylated affinity-labeled enzyme afforded, as a major radioactive product, a compound composed solely of aspartic acid and glucosamine at the molar ratio of 1:2. Peptic digestion afforded a single radioactive peptide cor39 responding to the sequence Asn-Thr-Gln-Ala-Thr-Asn-
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 248 16 شماره
صفحات -
تاریخ انتشار 1973